Introduction
Stage1 Study the
development of ITS sequence based molecular markers to distinguish Tribulus terrestris from
adulterants. It is medicinal plant in traditional medicine of many country, as Ayurveda,
Chinese, Korean.
Stage 2 Balasubramani,
Murugan, Ravikumar, and Venkatasubramanian (2010) explain
that angiosperm groups have nuclear ribosomal RNA genes and ITS (internal
transcribe spacer) sequences which are believed to be a suitable source
of evidence to determine phylogenetic relationships. Kumar and Verma (2012) study in comparative phylogenetic
analysis of medicinal plant Tribulus terrestris in Northwest India
revealed by RAPD.Stage 3 This paper study in distinction between Tribulus terrestris and adulterants because T. terrestris is one of the highly traded raw drugs and rare, it’s often adulterated. The development of DNA sequencing markers on RNA gene and ITS sequence can identify the real plant.
Stage 4 The objective of the this study was to develop specific markers for distinguishing T. terrestris and its adulterants.
Stage 5 This topic are use to develop specific markers for identification and authentication of raw drugs
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